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Auramine phenol stain is a stain used in clinical microbiology and histology to identify tuberculosis mycobacteria. There are two types of auramine phenol stains, 1 and 2 to stain mycobacterium species and cryptosporidium respectively. Both are fluorescent stains. The bacteria or the parasites appear brilliant greenish yellow against dark background.〔(【引用サイトリンク】url=http://microblog.me.uk/306 )〕 Mycolic acids of the mycobacteria keep this stain when decolorising with the acid alcohol.The method is more rapid and sensitive than ZN technique.〔(【引用サイトリンク】url=http://journals.sbmu.ac.ir/ijcid/article/download/198/143 )〕 ==Method== *Smears are prepared just like that for ZN staining *Stain with Auramine-Phenol for 20 mins *Rinse with water *Decolourise in acid alcohol *Rinse with water *Counterstain with 0.1% potassium permanganate for 30 seconds *Rinse and air dry〔(【引用サイトリンク】url=http://www.diasys.com/products/parasitology/fixatives-reagents-and-stains/parasitology-stains/ )〕 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Auramine phenol stain」の詳細全文を読む スポンサード リンク
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